The primary objective of this research is to identify trends for quantitative polymerase chain reaction (PCR) techniques, specifically the use of nascent digital PCR (dPCR) technology in comparison with the mature real-time quantitative PCR (qPCR) technology. The research strives to compare differences of current and future use between qPCR and dPCR; identify dPCR and dPCR technology brand use and future use trends; evaluate currently used qPCR and dPCR technology brands; identify influential factors contributing to qPCR and dPCR instrument selection; and determine for which applications and reactions researchers use qPCR and qPCR technologies.

Table Of Contents

TABLE OF CONTENTS

Research Background, Objectives, Methods, and Firmographics 6
•Frequency of qPCR and dPCR Instruments and Consumable Usage 13
Executive Summary and Implications 14
The Impact of Digital PCR and Real-time PCR 20
•Real-time versus Digital: Does Newer Mean Domination? 21
•Frequency of Performing qPCR and dPCR Assays: Real-time versus Digital 22
•Influencing Factors on Real-time PCR Selection 23
•The Impact of Real-time PCR Influential Factors on Digital PCR 24
•Cost Expectations for Real-time PCR Systems 25
•Cost Expectations for Real-time PCR Systems by Organization Types 26
•Cost Expectations Between Real-time PCR and Digital PCR 27
•Recognizing the Need for Improvements in the Real-time PCR Workflow 28
•Recognizing Dislikes in Current Real-time PCR Instruments 29
•Advantages of Digital PCR Over Real-time PCR 30
•Unmet Needs Regarding Digital PCR 31
•Reasons to Purchase Digital PCR—Verbatim Comments 32
•Summary: The Impact of Digital PCR on Real-time PCR 33
Quantitative (Real-time) PCR Usage Details 34
•Outsourcing Details for Real-time PCR 35
•Usage Details of Top Used Real-time PCR Brands/Models 36
•Mean Number of Real-time PCR Systems Currently Installed 43
•Usage Details of Future Bio-Rad Real-time PCR Users 45
Quantitative (Real-time) PCR Applications and Reactions Details 53
•Quantitative PCR Applications: Current and Future 54
•Anticipated Trend of the Number of Reactions on 2013 61
•Laboratories’ 2012 Budget for Real-time PCR Consumables 62
•Top Real-time PCR Product Suppliers Used Within the Past Year 63
•Overall Experience Ratings of Top Used Real-time PCR Product Suppliers 64
•Beliefs/Attitudes Toward Real-time PCR 65
Digital PCR Usage and Future Intention Details 69
•Usage Details of Digital PCR Brands/Models: Among Current dPCR Users 70
•Planned Digital PCR Brands Used: Among Future Users 71
The Frost and Sullivan Story 75

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