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Global Next-generation Sequencing Trends

  • September 2014
  • -
  • Frost & Sullivan
  • -
  • 116 pages

An End-user Perspective

The overall research objective was to capture and analyze global next-generation (NGS) sequencing trends and use. Most researchers outsource NGS technologies; however, in-house NGS technology use is expected to increase within the next 12 months. Data accuracy and operating costs are the top criteria for purchasing sequencing instruments. Disease-related research is the dominant use for NGS technologies. The popularity of RNA-Seq continues to surge, with half of the respondents planning to use this application in the next 24 months. The most significant bottleneck in the NGS workflow is the length of time spent analyzing data. The in-house annual budget for NGS data interpretation software is more than double that of those who outsource.

Research Background and Objectives

Research Background
Frost & Sullivan and the editors of The Scientist Magazine embarked on this study to capture and analyze global next-generation sequencing (NGS) trends and use.

Research Objectives
The overall research objective was to capture the current and planned use trends of NGS technology. Specifically, this research attempts to do the following:
-Compare the in-house and outsourcing use of NGS technology
-Capture the most important attributes in purchase decisions of sequencing instruments
-Evaluate use and satisfaction levels of NGS instruments, reagents, services, and bioinformatics providers
-Tap into the applications of NGS
-Determine laboratories’ NGS budgets and throughput needs
-Understand challenges and unmet needs in the NGS workflow

Table Of Contents

Global Next-generation Sequencing Trends
1. RESEARCH OBJECTIVES AND METHODS

Research Objectives and Methods
Research Background and Objectives
Methods
Sample Details

2. EXECUTIVE SUMMARY

Executive Summary
Outsourced Compared to In-house NGS—Current and Planned
Importance of Attributes in Purchasing Sequencing Instruments
Primary Research Area of NGS
Average Time Spent on Analyzing and Interpreting NGS Data
Annual Budget for Software for Analyzing and Interpreting NGS Data

3. IN-HOUSE AND OUTSOURCING USE RATES OF SEQUENCING TECHNOLOGIES

In-house and Outsourcing Use Rates of Sequencing Technologies
Technologies Used In-house—Current and Planned
Outsourced Technologies—Current and Planned

4. IN-HOUSE NEXT-GENERATION SEQUENCING DETAILS

In-house Next-generation Sequencing Details
Average Number of Currently Installed/Shared NGS Instruments
Average Number of Currently Installed/Shared Capillary Electrophoresis Sequencing Instruments
Importance of Attributes in Purchasing Sequencing Instruments
Top 10 Sequencing Instruments—Installation Details by Brands
Sequencing Instruments—Installation Details by Brands
Purchase Potential of New Upcoming Sequencing Technologies

5. NEXT-GENERATION SEQUENCING OUTSOURCING DETAILS

Next-generation Sequencing Outsourcing Details
Outsourcers of NGS—Current and Planned
Top Outsourced Commercial Service Providers—Current and Planned

6. NEXT-GENERATION SEQUENCING APPLICATIONS

Next-generation Sequencing Applications
Primary Research Area of NGS
Sequencing Applications—Current and Future

7. NEXT-GENERATION SEQUENCING REAGENTS

Next-generation Sequencing Reagents
NGS Reagents Providers
Overall Experience atings of the Most sed roviders

8. BIOINFORMATICS

Bioinformatics
Expected Sample Throughput for NGS Projects in 2014
Average Time Spent on Analyzing and Interpreting NGS Data
Average Number of Hours Spent on Analyzing and Interpreting NGS Data (continued)
Annual Budget for Software for Analyzing and Interpreting NGS Data
Per-sample Cost for NGS Interpretation
Top Companies Used for NGS Bioinformatics Purposes
Top Voted Providers of NGS Services
Sequencing Data Stored in the Cloud

9. DEMOGRAPHIC PROFILE

Demographic Profile
Involvement in the Use of NGS Technologies
Location of the Laboratory
Location of the Organization
Current Positon
Legal Disclaimer

10. APPENDIX—IN-HOUSE AND OUTSOURCING USE RATES OF SEQUENCING TECHNOLOGIES

Appendix—In-house and Outsourcing Use Rates of Sequencing Technologies
Technologies Currently Used In-house by Lab Location
Technologies Currently Used In-house by NGS Use
Technologies Planned to be Used In-house by Lab Location
Technologies Planned to be Used In-house by NGS Use
Technologies Currently Outsourced by Lab Location
Technologies Currently Outsourced by NGS Use
Technologies Planned to be Outsourced by Lab Location
Technologies Planned to be Outsourced by NGS Use

11. IN-HOUSE NEXT-GENERATION SEQUENCING DETAILS

In-house Next-generation Sequencing Details
Importance of Attributes in Purchasing Sequencing Instruments by Lab Location
Imporance of Attributes in Purchasing Sequencing Instruments by NGS Use
Sequencing Instruments—Currently Installed/Shared by Lab Location
Sequencing Instruments—Currently Installed/Shared by NGS Use
Sequencing Instruments—Planned to be Installed/Shared by Lab Location
Sequencing Instruments—Planned to be Installed/Shared by NGS Use
Sequencing Instruments—No Plans to Use by Lab Location
Sequencing Instruments—No Plans to Use by NGS Use
Purchase Potential of New Upcoming Sequencing Technologies by Lab Location
Purchase Potential of New Upcoming Sequencing Technologies by NGS Use

12. NEXT-GENERATION SEQUENCING OUTSOURCING DETAILS

Next-generation Sequencing Outsourcing Details
Currently Used Outsourcers of NGS by Lab Location
Currently Used Outsourcers of NGS by NGS Use
NGS Outsourcers Planned to be Used by Lab Location
NGS Outsourcers Planned to be Used by NGS Use
Currently Outsourced Commercial Service Providers
Commercial Service Providers Planned to be Outsourced

13. NEXT-GENERATION SEQUENCING APPLICATIONS

Next-generation Sequencing Applications
Primary Research Area of NGS by Lab Location
Primary Research Area of NGS by NGS Use
Currently Conducted Sequencing Applications by Lab Location
Currently Conducted Sequencing Applications by NGS Use
Sequencing Applications Planned to be Conducted by Lab Location
Sequencing Applications Planned to be Conducted by NGS Use

14. NEXT-GENERATION SEQUENCING REAGENTS

Next-generation Sequencing Reagents
NGS Reagents Providers by Lab Location
NGS Reagents Providers by NGS Use

15. BIOINFORMATICS

Bioinformatics
Average Time Spent on Analyzing and Interpreting NGS Data by Lab Location
Average Time Spent on Analyzing and Interpreting NGS Data by NGS Use
Companies Used for NGS Bioinformatics Purposes
Best Providers of NGS Bioinformatics Services

16. DEMOGRAPHIC PROFILE

Demographic Profile
Involvement in the Use NGS Technologies by Lab Location
Involvement in the Use NGS Technologies by NGS Use

17. THE FROST and SULLIVAN STORY

The Frost and Sullivan Story
Value Proposition—Future of Your Company and Career
Global Perspective
Industry Convergence
360º Research Perspective
Implementation Excellence
Our Blue Ocean Strategy

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