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  4. > Library Preparation and Target Enrichment for Next Generation Sequencing Product Markets

Library Preparation and Target Enrichment technologies are a necessary part of the sequencer's use in clinical applications. As the sequencer technologies become cheaper, they have been increasingly moving into more labs and new types of research projects in a virtuous cycle. The capabilities of next-generation sequencer (NGS) systems, along with the growing availability of vast amounts of annotated sequence data, have permitted life science researchers to undertake a much wider range of experiments. The diverse needs of these experiments have resulted in a high demand for library preparation and target enrichment products.

This report, Library Preparation and Target Enrichment for Next Generation Sequencing Product Markets contains valuable information on the current state of LP/TE for next generation sequencing including:
Market Size and Forecasts for Library Prep and Target Enrichment
Market Share and Competitive Analysis
Over 340 Deals, Collaborations and Partnerships Since 2010 involving LP/TE.
Library Preparation and Target Enrichment Products Launched Since 2012
Important Trends in the Industry
Litigation and Intellectual Property Review
Analyst's Conclusions and Recommendations

DNA and RNA sequencing are becoming more central components of life science research. In addition to their prices dropping, the technologies continue to rapidly improve and become more user-friendly. The applications of sequencing span a broad range of industries and specializations, but the focus has mostly been life science research-related. As the science becomes more established, various clinical applications have become proven, and there are certain areas such as cancer where there are expected to be rapidly growing benefits in the near future.

The NGS systems are capable of a wide range of applications, and labs have taken advantage of their flexibility. The same system is often used for five or ten distinct applications. The NGS systems also have specific constraints on the type of sample that can be loaded, with regard to fragment size, adapters, and the like. The protocols can sometimes require several steps. These factors have created a market for a large range of products catering to all of the different variations of these numerous applications.

As part of its coverage of the report, several major competitors are profiled. Companies profiled in the report are

Agilent Technologies
F. Hoffmann-La Roche AG
Fluidigm
Illumina
Pacific Biosciences
Qiagen
Thermo Fisher Scientific

Table Of Contents

Library Preparation and Target Enrichment for Next Generation Sequencing Product Markets
CHAPTER ONE: EXECUTIVE SUMMARY
Introduction
Background
Trends
Market Growth in Library Preparation and Target Enrichment
Acceleration of Product Introductions
A Period of Rapid Adoption, But Lower Prices
Likelihood of Longer-Term Challenges
Rapid Growth Overall, But Intense Competition
Customers’ Research Models Evolving
Scope and Methodology
Applications and Definitions
Applications
Experiments
Promising Opportunities
Gaps in Scientific Knowledge
High Priority Research Areas
DNA Fragmentation
DNA Size Selection
Target Enrichment
CHAPTER TWO: TRENDS
Trends Relating to Sequencing, Target Enrichment and Library Prep
Rapid Adoption of Smaller Systems Driving Applications
Decline in Array-Based Target Enrichment Techniques
Stability Despite Possibility of Disruptive Technologies
Sequencer Sales Continue Dramatic Growth
Inherited Diseases and Cancer Driving Clinical Adoption
Regulatory Situation Unclear But Evolving Somewhat
CHAPTER THREE: PRODUCT LAUNCHES 2012-2105
2015 Product Introductions
BGI NGS System Launch
Illumina HiSeq X Five
2014 Product Introductions
Fluidigm Porducts
Eznymatics Archer Fusion Plex
Illumina Launches New HiSeq X Ten Reagent Kits
Roche SeqCap RNA
Agilent Sure Select Focused Exome
Beckman Coulter NEBNext UltraDirectional RNA
Fluidigm Single-Cell Exome Sequencing Workflow
Clear Seq AML
Lexogen QuantSeq3
Agilent SureSelect Clinical Research Exome
NextFlex Cell Free DNA
Enzymatics Archer FusionPlex Sarcoma Panel
Multiplicom STARGARDT MASTR
Qiagen GeneRead DNAseq V2
Agilent SureSelectQXT
HTG Molecular Diagnostics EdgeSeq miRNA
NuGEN Ovation Custom Target Enrichment System
Illumina V4 Reagents Kits for HiSeq
Illumina VeriSeq
Kapa Biosystems Hyper Prep Kit
Lexogen QuantSeq 3 mRNA-seq Library Prep kit,
Life Technologies Ion Chef
NuGEN Insert Dependent Adaptor Cleavage
Qiagen QIAexpert
Guardant Health 360 Test
Integrated DNA Technologies Launches Panels
Kapa Biosystems Adds Amplification Primers
Zymor Research Launches PicoMethyl Seq Library Prep
and RRHP 5hmC Library Prep kits.
Bioo Scientific NextFlex Dual
Covaris TruXtrac High Throughput FFPE
Enzymatics Archer ALK/RET/ROS1
epMotion for TruSeq
Life Technologies HID-Ion AmpliSeq
Qiagen REPLI-g WTA
RainDance ThunderBolts Cancer Panel
Rubicon Genomics PicoPlex DNA Seq
Illumina Next Seq 500
2013 Product Introductions
2012 Product Introductions
CHAPTER FOUR: LIBRARY PREPARATION AND TARGET ENRICHMENT MARKET
Revenues Forecast
Cost of Sequencing a Genome
Library Preparation and Target Enrichment Market
Growth Drivers
Illumina Consumables Revenues as Bellwether
CHAPTER FIVE: MARKET SHARE AND COMPETITIVE ANALYSIS
Introduction
Sequencer System Battles Turn Somewhat Calm
Library Preparation and Target Enrichment Market Shares
Smaller Companies Experiencing Mixed Success
CHAPTER SIX: INTELLECTUAL PROPERTY AND LITIGATION
Important Litigation and IP Developments
Sequenom and Other NIPT Companies Suing Each Other
Sequenom Shareholder Sues Over Price “Capping�
Class Action Shareholder Lawsuit Filed Against Pacific Biosciences
Helicos’ Patent Infringement Suit Against PacBio, Life Technologies, and Illumina
Illumina’s Two Lawsuits Against Complete Genomics for Infringement
Life Technologies and Illumina Dispute Over Amplification on Solid Surfaces
Life Technologies Files Infringement Suit Against Promega
Enzo Biochem Disputes CalTech Sequencing Patents
Azco Biotech Sues Qiagen and Intelligent Bio-Systems
Intelligent Bio-Systems (Qiagen) and Columbia University Sue Illumina
for Infringement
LabCorp’s Esoterix Sues Life Technologies
Genetic Technologies Sues Several Firms for Infringement of Haplotype
Determination Technique
Illumina Sues Life Technologies Over Ion Torrent Systems
Affymetrix Dismisses Suit Against Pacific Biosciences and Former Employees
Life Technologies Defeats VisiGen Stakeholders After Arbitration
Catalyst Assets Sues Life Technologies for Infringement
Patent Interference Between Life Technologies and Pacific Biosciences
LadaTech Sues Illumina Over DNA Amplification Patent
Illumina Files ‘841 Patent Infringement Suit Against Affymetrix
Affymetrix and Illumina Settle Case Over ‘243, ‘432, ‘531, ‘365, and ‘716
Applied Biosystems and Illumina Claims and Counter-claims Over Sequencingby-
Ligation Both Unsuccessful
Fluidigm and Applied Biosystems Agree to End Case
Beckman Coulter and Applied Biosystems Settle Outstanding Legal Disputes
Cepheid and Idaho Technology Settle Dispute Over PCR Patents
Huang v. CalTech
Applied Biosystems and Amersham plc (GE Healthcare) Settle Sequencing
Patent Litigation
Early ABI (Life Technologies) Patents
CHAPTER SEVEN: DEALS ANALYSIS -340 COLLABORATIONS, MERGERS, PARTNERSHIPS SINCE 2010
List of Deals Involving NGS, LP and TE, Feb 2010 to Jan 2015
CHAPTER EIGHT: CORPORATE PROFILES
Agilent Technologies
F. Hoffmann-La Roche AG
Fluidigm
Illumina
Pacific Biosciences
Qiagen
Thermo Fisher Scientific
CHAPTER NINE: CHALLENGES AND STRATEGIC RECOMMENDATIONS
Challenges
Risks
Use of Services
Shortcomings and Pitfalls
Low Barriers
LDT
Prices
Restraints on Adoption
Strategic Recommendations
Partnership Strategy
Product Development Strategy
Preparation Needed
Role of Services
Role of Data
Global Market Strategy


LIST OF EXHIBITS
CHAPTER ONE: EXECUTIVE SUMMARY
Figure 1-1: Market Growth in Target Enrichment and Library
Preparation, 2014 to 2019
CHAPTER THREE: PRODUCT LAUNCHES 2012-2015
Table 3-1: Products for Library Preparation and Target Enrichment
CHAPTER FOUR: LIBRARY PREPARATION AND TARGET ENRICHMENT MARKET
Figure 4-1: Cost of Sequencing a Genome
Table 4-1: Library Preparation and Target Enrichment Market
Figure 4-2: Library Preparation and Target Enrichment
Revenues, 2014 and 2019
Table 4-2: Library Preparation and Target Enrichment Growth Rate
Figure 4-3: Library Preparation and Target Enrichment Growth Rate
Table 4-3: Revenues by Region, 2014
Figure 4-4: Revenues by Region, 2014
Table 4-4: Illumina Quarterly Consumables Revenues (2012-2014)
Figure 4-5: Illumina Quarterly Consumables Revenues (2012-2014)
Table 4-5: Illumina Annual Consumables Revenues for Sequencing and
for Other Purposes (2012-2014)
Table 4-6: Illumina Consumables Growth Rates for
Seqeuncing Consumables (2012-2014)
Figure 4-6: Illumina 2012 Sequencing Consumables Share
Figure 4-7: Illumina 2014 Sequencing Consumables Share
CHAPTER FIVE: COMPETITIVE ANALYSIS
Table 5-1: Library Preparation and Target Enrichment Market Shares
Figure 5-1: Library Preparation and Target Enrichment Market Shares
CHAPTER SEVEN: 340 DEALS
Table 7-1: List of Deals Involving NGS, LP and TE, Feb 2010 to Jan 2015

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