This comprehensive review of the DNA and RNA probe technology includes modern principles of probe preparation, various test formats, labeling techniques, and numerous amplification methods.

Contains 60 pages


Table Of Contents

Table of Contents

1. Basic Principles
2. Probe Preparation
3. DNA Probe Test
a. Sample Preparation
b. Hybridization
c. Separation
d. Detection/Measurement
4. Test Formats
a. Filter Hybridization
b. Southern Blot
c. Northern Blot
d. In Situ Hybridization
e. Others
5. Labeling Techniques
6. Amplification Methods
Polymerase Chain Reaction
Temperature Cyclers
PCR Variations
ImmunoPCR
QCPCR
DAPPCR
Strand Displacement Activation
TMA
Ligase Chain Reaction
Branched DNA
Hybridization Protection Assay
NucleicAcid SequenceBased Amplification
SelfSustained Sequence Replicase
Others
Ampliprobe
CAR
CAS
CPT
Dendritic Polymer Technology
ISOCR
LAT
Probe Networks
RAMP
Repair Chain Reaction
Rolling Circles
Sequence Independent Gene Amplification
Sequence Initiation Reaction
SISPA

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