DNA Sequencing and PCR Markets

  • November 2013
  • -
  • TriMark Publications
  • -
  • 176 pages

DNA sequencing and polymerase chain reaction (PCR) are two major technology platforms of value in life science research, drug discovery and clinical diagnostics. The markets for sequencing are extremely competitive, driven by rapid technological advancements and aggressive price wars. Next-generation sequencing presents an exciting area of growth for life science tool vendors, including those providing sequencers, ancillary instrumentation, reagents and software. This TriMark Publications report provides an in-depth analysis of key technology and market trends in the high-throughput sequencing space while analyzing the main drivers of growth in markets for PCR products and applications. The study provides a thorough overview of DNA sequencing and PCR, including applications, opportunities, technological trends, and market share and dynamics with a particular focus on the U.S, Japan, European, and Brazil, Russia, India and China (BRIC) markets. This report also analyzes almost all of the companies known to be marketing, manufacturing or developing DNA sequencing and PCR products in the U.S. and worldwide. Each company is discussed in extensive depth with a section on its history, product line, business and marketing analysis, and a subjective commentary of the company's market position. Detailed tables and charts with sales forecasts and market data are also included.

Table Of Contents

TABLE OF CONTENTS

1. Overview 7
1.1 Statement of Report 7
1.2 About This Report 7
1.3 Scope of the Report 7
1.4 Methodology 8
1.5 Executive Summary 9

2. Introduction to DNA Sequencing and PCR Technology 14
2.1 Science Overview: Introduction to DNA 14
2.2 DNA Sequencing and PCR Applications Space 18
2.2.1 DNA Sequencing Technologies 19
2.2.2 DNA Sequencing Applications 22
2.2.3 Proteomics 23
2.2.4 Products for the Genomics Market 23
2.3 The Impact of the Human Genome Project on Molecular Biology 23
2.4 Impact of DNA Sequencing Techniques on Biotechnology, Pharmaceutical and
Healthcare
Industries 24
2.5 Molecular Diagnostics in the Post-Genomic Era—The Role of SNPs 24
2.6 Advances in Molecular Diagnostic Technologies 25
2.7 The Use of PCR Technology 26
2.8 Genetic Variability and Disease 28

3. DNA Sequencing Technologies and Market Analysis 30
3.1 Overview of the Sequencing Market 30
3.1.1 Market Opportunity 31
3.1.2 Highlights of DNA Sequencing Market 33
3.1.3 Genomic Analysis Market Development 34
3.1.4 Companies Offering Sequencing Technology Platforms 36
3.1.5 Competition 40
3.1.6 Markets for DNA Sequencing 42
3.1.7 Market Opportunity 44
3.1.8 Market Dynamics 44
3.1.9 Overview of Current DNA Sequencing Technology Platforms and Players 45
3.1.10 Limitations of Current Sequencing Technologies 51
3.1.11 Next-Generation Sequencing Software 53
3.1.12 Sequencing Services Industry: Market Size and Outlook 60
3.2 Overview of the Life Science Marketplace 64
3.2.1 Research Laboratories 68
3.2.2 Market Share 69
3.2.3 Paradigm Shift in Drug Discovery and Development 70
3.2.4 Systems Biology and the Need for More Effective Tools 70
3.2.5 Molecular Medicine and the New Therapeutics 70
3.2.6 Pharmacogenomics Using Genotyping and Phenotyping Clinical Biomarkers 71
3.3 The Market for Genetic Analysis Products 71

4. PCR and DNA Amplification Technologies and Markets 74
4.1 Technology Overview 74
4.1.1 Nucleic Acid Amplification Methods 74
4.1.2 Overview of PCR Technology 76
4.1.2.1 PCR Components 77
4.1.2.2 Primers for PCR 77
4.1.2.3 Equipment for PCR 77
4.1.2.4 Sources of DNA for PCR 78
4.1.2.5 PCR Primer Sequence 79
4.1.2.6 Thermostable DNA Polymerases 80
4.1.2.7 PCR Protocols 80
4.1.3 Variations of PCR 82
4.1.4 Detection of Amplified Gene Products 84
4.1.4.1 High Resolution Melting Analysis 85
4.1.4.2 Microparticle-Based Flow Cytometry 85
4.2 Overview of Current PCR Technology Platforms and Players 86
4.2.1 Future Directions for PCR Technology 88
4.3 DNA Amplification Market 88
4.3.1 Market Size and Growth Projections in DNA Amplification Instrumentation,
2010-2018 89
4.3.1.1 U.S. Market 90
4.3.1.2 European Market 90
4.3.2 Market Share of Key Players in DNA Amplification 90
4.3.3 Markets for Emerging Amplification Technologies 91
4.3.4 The Drivers for Amplification Technologies 94
4.4 Instrumentation Outlook for the Life Sciences Industry 94
4.5 Products for the Genomics Market 95

5. Technical Trends in Nucleic Acid Analysis 96
5.1 Amplification of Nucleic Acids 96
5.1.1 Overview 96
5.1.2 Quality Control Issues in PCR 96
5.1.3 Detection of the Amplified Product 97
5.1.4 Novel Nucleic Acid Amplification Techniques 99
5.2 Alternatives to PCR Amplification 99
5.2.1 Locked Nucleic Acids (LNAs) 99
5.2.2 Multiplexed Nuclease-Protection Assay and ArrayPlate 100
5.2.3 Biosensor Technologies 101
5.2.3.1 DNA Hybridization Biosensor Chips 101
5.2.3.2 PNA-Based Biosensors 102
5.3 Microplate Readers 102
5.4 DNA Sequencing Technologies 102
5.4.1 Capillary-Based Sequencers 102
5.4.2 Pyrosequencing 103
5.4.3 High-Throughput Sequencing 103
5.4.4 Emerging Technologies 106
5.4.5 Emerging Markets for Next-Generation Sequencing 107
5.5 Nucleic Acid Sample Prep Platforms 109
5.6 RNA Isolation Kits 110
5.7 DNA Microarrays 111
5.7.1 The Technique 111
5.7.2 The Market 111
5.7.3 Advantages of Microarrays 113
5.7.4 Next-Generation Sequencing vs. Microarrays 114

6. Corporate Directory 115
6.1 Abbott Laboratories 115
6.2 Affymetrix, Inc. 115
6.3 Agilent Technologies, Inc. 117
6.4 AgriGen Biotech Pty Ltd. 118
6.5 Ambry Genetics 118
6.6 Avesthagen Gengraine Technologies Pvt. Ltd. 118
6.7 BD Biosciences 119
6.8 Beckman Coulter Genomics, Inc. 119
6.9 BGI Life Tech 119
6.10 BioCat GmbH 120
6.11 BioChain, Inc. 120
6.12 Biogazelle 121
6.13 bioMerieux 121
6.14 Bioneer 122
6.15 Bio-Rad Laboratories 122
6.16 BioServe 123
6.17 Cepheid 123
6.18 Commonwealth Biotechnologies, Inc. 124
6.19 Complete Genomics 125
6.20 Comprehensive Biomarker Center GmbH 125
6.21 Danaher Corporation 126
6.22 DNA LandMarks 127
6.23 DNAVision 128
6.24 DNASTAR, Inc. 128
6.25 ELITech Molecular Diagnostics 128
6.26 Enigma Diagnostics Limited 128
6.27 Enzo Biochem 129
6.28 Eppendorf AG 130
6.29 Eurofins Medigenomix GmbH 130
6.30 Exiqon 130
6.31 FASMAC Co., Ltd. 131
6.32 Fluidigm Corporation 131
6.33 GE Healthcare 132
6.34 GenapSys, Inc. 133
6.35 GeneticTechnologies Limited 133
6.36 GeneWorks Pty. Ltd. 134
6.37 Genia 134
6.38 Genisphere, Inc. 134
6.39 Genovoxx 135
6.40 GnuBIO, Inc. 135
6.41 GVK BIO 135
6.42 Helicos BioSciences Corporation 136
6.43 High Throughput Genomics, Inc. 136
6.44 Hokkaido System Science 136
6.45 Hologic, Inc. 137
6.46 Hy Laboratories Ltd. 138
6.47 Illumina, Inc. 139
6.48 Inqaba Biotechnical Industries Ltd. 140
6.49 IntegenX, Inc. 141
6.50 Integrated DNA Technologies 141
6.51 Kreatech Holding BV 141
6.52 LaserGen, Inc. 142
6.53 LI-COR Biosciences 142
6.54 Life Technologies Corporation 142
6.55 Lucigen Corporation 143
6.56 Luminex Corporation 144
6.57 Meridian Biosciences 146
6.58 Microsynth AG 146
6.59 MilleGen SA 146
6.60 Monoquant 147
6.61 MWG Biotech AG 147
6.62 Nabsys 147
6.63 NanoString Technologies 148
6.64 NobleGen Biosciences 148
6.65 OncoDNA SA 149
6.66 Orchid Cellmark 149
6.67 Oxford Nanopore Technologies Ltd. 149
6.68 Pacific Biosciences 150
6.69 PamGene 150
6.70 PerkinElmer, Inc. 150
6.71 PPD, Inc. 152
6.72 PrimmBiotech 152
6.73 Promega 152
6.74 QIAGEN N.V. 152
6.75 RainDance Technologies 154
6.76 Research Biolabs Pte 154
6.77 Roche Diagnostics 154
6.78 Rubicon Genomics, Inc. 155
6.79 Saturn Biotech Limited 155
6.80 SeeGene 155
6.81 Sequenom, Inc. 155
6.82 Sequetech 156
6.83 SeqWright 157
6.84 Shanghai Sangon Biological Engineering Technology and Services Co., Ltd.
157
6.85 Shimadzu Biotech 157
6.86 Siemens AG 157
6.87 SolGent, Inc. 158
6.88 Stratos Genomics, Inc. 158
6.89 TATAA Biocenter 158
6.90 Thermo Fisher Scientific, Inc. 158
6.91 Third Wave Technologies, Inc. 159
6.92 Wellcome Trust Sanger Institute 161
6.93 ZS Genetics 161

7. Market Trends and Forecasts 162
7.1 Market Dynamics 162
7.2 Future Considerations for Molecular Diagnostics 162
7.2.1 How Genomics is Transforming Pharmaceutical Randamp;D 163
7.2.2 Genetic Markers for Disease 163
7.2.3 NAT and Personalized Medicine 164
7.2.4 Growth Areas of Molecular Diagnostics 164
7.3 RNA Detection 164
7.4 Ramification Amplification Method 165
7.5 Invader Assays/Hologic 165
7.6 Molecular Beacons 166
7.7 PCR-on-a-Chip 166
7.8 ChIP Sequencing 167
7.9 Methylation Analysis 169
7.10 Digital Polymerase Chain Reaction (dPCR) 170
7.11 Personal or Desktop Sequencers 171

8. Major Activities in DNA Sequencing and PCR Markets 173
8.1 Product Innovations/Launches in DNA Sequencing and PCR Industries 173
8.2 Mandamp;A, and Other Major Activities in the DNA Sequencing and PCR Industries 175


INDEX OF FIGURES

Figure 2.1: Segment of Double-Stranded DNA Showing the Base-Pair Relationship 16
Figure 3.1: Cost of Sequencing a Human Genome, 2001-2013 31
Figure 3.2: Market Share in the Sequencing Industry, by Application, 2012-2017
33
Figure 3.3: Number of Sequencing Machines by Continent, 2013 35
Figure 3.4: Factors influencing Researchers’ Choice of Next-Generation
Sequencing Platform 36
Figure 3.5: Next-Generation Sequencing Platforms Market Share, 2013 41
Figure 3.6: Applications for Next-Generation Sequencing in the Research
Laboratory 43
Figure 3.7: Consumables in Research Laboratories: Market Breakdown and
Corresponding Growth Rates,
2012-2015 65
Figure 3.8: The Market for Genetic Analysis Tools 72
Figure 4.1: Cepheid’s I-CORE Module 76
Figure 4.2: Market Share for Thermal Cycler Manufacturers 89
Figure 5.1: Nanopore Sequencing 107
Figure 7.1: Market Shares for Benchtop Sequencers, 2013 172
Figure 7.2: Evolution of the Market for Benchtop Sequencers, Predicted Market
Shares for 2015 172


INDEX OF TABLES

Table 2.1: First- and Second- (Next-) Generation Sequencers 21
Table 2.2: Third- and Fourth-Generation Sequencing Platforms 21
Table 2.3: Applications of Next-Generation Sequencing 22
Table 2.4: Comparative Strengths of qPCR and dPCR 28
Table 3.1: Global Market for Next-Generation Sequencing, 2012-2018 32
Table 3.2: Sequencing Platforms on the Market and in Development 37
Table 3.3: DNA Sequencing Market Players 38
Table 3.4: Major DNA Product Companies in Europe 38
Table 3.5: Major DNA Product Companies in Asia and Australia 39
Table 3.6: Major DNA Product Companies in Japan 39
Table 3.7: Major DNA Product Companies in Rest of the World 39
Table 3.8: Comparison on Pricing of Current NGS Platforms, 2013 46
Table 3.9: Number of Sequencers Currently in Use at Academic and Sequencing
Centers Worldwide, by
Platform 47
Table 3.10: Comparison of Established Genomic Analysis Technologies 52
Table 3.11: Integrated Software Solutions for Next-Generation Sequencing 56
Table 3.12: Software Solutions for Resequencing 56
Table 3.13: Software Solutions for De novo Alignment/Assembly 57
Table 3.14: Software Solutions for SNP/Indel Discovery 57
Table 3.15: Software Solutions for Genome Annotation 58
Table 3.16: Software Solutions for ChIP-Sequencing and Methylome-Sequencing 58
Table 3.17: Software Solutions for Transcriptomics 58
Table 3.18: Tools for the Analysis of Next-Generation Sequencing Data 59
Table 3.19: Sequencing Services Market Size, 2010-2018 61
Table 3.20: Top 30 Genome Centers Worldwide 61
Table 3.21: Genome Centers by Country 62
Table 3.22: Frequently Used Life Science Techniques 66
Table 3.23: Reagents Used by Life Science Researchers 66
Table 3.24: The Most Commonly Used Instruments and Apparatus in Life Science
Research 67
Table 3.25: The Most Widely Reported Categories of Software in Life Science
Research 67
Table 3.26: Global Market Size for Life Science Research Tools, 2011-2018 67
Table 3.27: Key Bioassay Technologies in the Life Sciences Industry 69
Table 4.1: Nucleic Acid Amplification Methods 74
Table 4.2: Properties of Various Acid Amplification Techniques 76
Table 4.3: Global Market Size for DNA Amplification Instrumentation, 2010-2018
89
Table 4.4: Global Market Size for Reagents and Consumables in the DNA
Amplification Segment,
2010-2018 90
Table 4.5: Market Share of Key Players in qPCR Enzymes and Supplies 91
Table 4.6: Leading Manufacturers of Thermal Cyclers and Market Share 91
Table 4.7: High Growth in the Global Market for Digital PCR, Market Size,
2012-2017 92
Table 4.8: Commercially Available Digital PCR Platforms 93
Table 5.1: Next-Generation Sequencing Platforms 104
Table 5.2: Comparative Benefits of Next-Generation Sequencing over Microarray
Technology 113
Table 8.1: The Key Bioassay Technologies in the Life Sciences Industry 173

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