Next Generation Sequencing for Life Science RandD: Advancing to the $1,000 Genome

Next Generation Sequencing for Life Science RandD: Advancing to the $1,000 Genome

This report examines the emerging, fast-growing worldwide market for next-generation genome sequencing systems and services. The makers of the new sequencing systems are competing in the race to drive down the cost of sequencing a human genome to $1,000 or less. At that cost, life scientists will be able sequence human genomes at the massive scale needed to better understand human variation and help open the gateway to personalized medicine.

The first wave of the commercial high-throughput genome sequencers has seen a rapid uptake from life science research customers at genome centers and research service providers from North America, Europe and Asia. This study looks at the basic technologies that these new DNA sequencers use and what kinds of applications and research projects that the scientists are doing.

The new platforms can help accelerate the research capabilities of basic academic medical research, pharmaceutical and biotech drug discovery and diagnostic developments. The report also studies the research service providers that outsource to pharma and biotech companies because the technologies might help them become more productive faster.

This report is for business executives, entrepreneurs, venture capitalists, investors, product managers, research managers, business development managers, and other decision makers. The next-generation genome sequencing products and services discussed include technologies from Illumina, Roche/ 454, Applied Biosystems, Danaher Motion, Helicos Biosciences, Pacific Biosciences and others.

This study discusses its interesting topics with key findings. The new data presented estimates that the total next-generation genome sequencing market size will reach $484 million in 2008 and grow at a high rate. It reviews the activities of 18 companies. The report uses 38+ figures and tables to illustrate the findings. The study features convenient tables that help the reader scan the details of key trends, market segmentation, forecasts, M&As, alliances, grant funding, patents and the like. 
 

Executive Summary

1. Overview of the Genome Sequencing Market for R&D

1.1 Overview of Current and Next-Generation Genome Sequencing for R&D

Figure 1. The Next-Generation Genome Sequencing Market Landscape

1.1.2 Genome Sequencing Equipment Systems

Current Sanger-based CE Sequencing Systems

Next-Generation Sequencing Systems (Commercial)

Next-Generation Sequencing Systems (In Development)

1.1.3 Bioinformatics Software

Table 1. Sequencer Vendor Provided Software(Source, NG Seq. Platform, Name)

Table 2. Selected Commercial Software (Source, Name, Comment)

Table 3. Selected Open Source Short-Read Assembly Software (Source, Name, Comment)

1.1.4 Genome Sequencing Organizations

Academic Genome Sequencing Centers

Academic/ Government Core Labs

Commercial Service Providers

1.1.5 IT Infrastructure and Applications

IT Infrastructure Vendors and Solutions

1.1.6 Others

1.2 Next-Gen. Genome Sequencing Applications & Projects for R&D

1.2.1 Academic Life Science Applications

1.2.2 Pharma/ Biotech Drug Discovery R&D

1.2.3 Clinical Diagnostic Applications and Projects

1.2.4 Public Health Applications

1.2.5 Emerging ‘Consumer Genomics’ Applications

1.2.6 Agricultural/ Veterinary Applications

1.2.7 Environmental (Metagenomics) Applications

1.2.8 Other Related Applications

2. Next-Gen. Genome Sequencing Technology Developments

2.1 Government and Private Next-Generation DNA Sequencing Initiatives

2.1.1 The NIH’s 2004 Proposal for Sequencing the Genome for $1,000

Table 4. Next-Generation Sequencing Tech. Grants Awarded by NHGRI 2004-2007 (Grant Year, Grant Goal, Organization, Next-Gen.Sequencing Technology Development Grants, Grant Amount, Term (Yr), Teams)

2.1.2 The $10 Million Archon X PRIZE for Genomics

Table 5. Competing Teams for the X PRIZE for Genomics

2.2 Developing Technologies for the Next Five-Years and Beyond

2.2.1 Near-Term Time Line Goal

2.2.2 $1,000 Genome Time Line Goal

2.3 Sequencing Technologies for the $100,000 Genome

Sample Preparation

Sequencing

Image Analysis

2.3.1 Roche/ 454 Life Sciences, Inc. GS FLX and XLR

Massively Parallel Sequencing by Synthesis (Pyrosequencing)

Figure 2. Roche/ 454 GS FLX DNA Sequencer

2.3.2 Illumina, Inc. Genome Analyzer II - Sequencing by Synthesis

Figure 3. Illumina Genome Analyzer

2.3.3 Applied Biosystems Inc. SOLiD - Polony Sequencing by Ligation

Figure 4. The ABI SOLiD Sequencing

2.3.4 Danaher Motion Polonator G.007 - Open Source Polony Sequencing System

Figure 5. The Danaher-Church Polonator G.007

2.3.5 Helicos BioSciences Inc. HeliScope - Single-Molecule Sequencing

Figure 6. Helicos HeliScope Sequencer

2.4 Sequencing Technologies for $1,000 Genome

2.4.1 Pacific BioSciences, Inc. Real Time Single-Molecule Sequencing

Figure 7. Simultaneous & Continuous Excitation & Detection in a ZMW

2.4.2 ZS Genetics Direct Electron Microscope Sequencing

Figure 8. Overview of the ZSG Sequencing Process

3. Key Alliances, M&As and IP

3.1 Summary of Key Alliances and M&As

3.1.1 Definitions of Alliance and M&A Deal Types

M&As

Development and Supplier Partnerships

IP Licensing Agreements

3.1.2 Highlights of Key Alliances and M&As

Table 6. Summary of Key Alliances and M&As, Mar/2005 to Jun/2008

3.1.3 Data Tables for Alliances and M&As

Table 7. Key Alliances and M&As Jan to Jun 2008 (Date, Technology, Alliance Type, Buyer, Seller, Comment)

3.2 IP, Business Development and Challenges

3.2.1 Summary of Key Patents Involving Sequencing Technologies

Table 11. NGS Related US Patents Issued to Orgs., 2007 to May/2008

3.2.2 The Business Value of IP

Table 12. Key Acquisitions in Next-Generation Sequencing.(Acquirer, Target Company, Comment, Value $M)

3.2.3 IP Challenges

4. The Market and Outlook for Next-Generation Genome Sequencing

4.1 The Total Worldwide Market Size, 2007-2012

Figure 9. Total Next-Gen. Sequencing Tools & Service Market 2007-2012

Table 13. Total Next-Gen. Sequencing Tools & Service Market 2007-2012

4.2 Market Size by Products & Services

4.2.1 Methodology and Definitions

4.2.2 Next-Gen. Sequencing Tools & Service Market By Product & Service Segments

Figure 10. Next-Gen. Sequencing Tools & Service Market By Product & Service Segments2007-2012

Table 14. Next-Gen. Sequencing Tools & Service Market By Product & Service Segments 2007-2012

4.2.3 Next-Gen. Sequencing Tools & Service Market By Product & Service Segment Shares, 2008 & 2012

Figure 11. Next-Gen. Sequencing Tools & Service Market By Product & Service Segment Shares, 2008 & 2012

Table 15. WW Market by Major Segments & Shares, 2008 & 2012

Figure 12. Next-Gen. Sequencing Tools & Service Market By Application Segments

Table 16. Next-Gen. Sequencing Tools & Service Market By Application Segments

4.3 Market by Customer Segments

Table 17. Next-Gen. Sequencing Tools & Service Market By Customer Segments

Figure 13. Next-Gen. Sequencing Tools & Service Market By Customer Segments

4.4 Market by WW Regions

Figure 14. Next-Gen. Sequencing Tools & Service Market By WW Regions

Table 18. Next-Gen. Sequencing Tools & Service Market By WW Regions

5. Activities of the Firms Offering Next-Generation Genome Sequencing Products & Services

5.1 NG Seq. Commercial Equipment

5.1.1 - 5.1.4 (Total 4 companies)

A. Contact Information

B. Key Business Facts

C. Company Overview, Technology and Key Products

D. Alliances, Strategy, Mergers and Acquisitions

E. Patents, Milestones, Funding, and Other Findings

5.2 NG Seq. Equipment Developers

5.2.1 - 5.2.4 (Total 4 compnies)

Same as above-mentioned research items

5.3 Consumer Genomics Services

5.3.1 - 5.3.4 (Total 4 companies)

Same as above-mentioned research items

5.4 Contract Genomic Research Services

5.4.1 - 5.4.4 (Total 4 companies)

Same as above-mentioned research items

5.5 Software

5.5.1 (Total one company)

Same as above-mentioned research items

6. Conclusions and New Business Opportunities

6.1 Likely Next-Gen. Genome Sequencing Success and Failure Factors

6.1.1 The Human Genome Project Cost $3.5 Billion and 13 Years Effort

6.1.2 Advances in Technology Enable Emerging Next-Gen. Sequencers.

6.1.3 Government Technology Initiative - The $100, 000 and $1,000 Genomes

6.1.4 Private Technology Initiative - The X PRIZE for Genomics Fosters Race to Reach the $10,000 Genome

6.1.5 Key M&As Enabled Commercial Next-Gen. Sequencing Platforms

6.1.6 Barriers to Market Entry Are Getting More Challenging

6.1.7 Next-Generation Sequencers Output Enormous Amounts of Data

6.1.8 Consumers Want Their Personal Genomes

6.1.9 Known Individuals are Getting Their Genomes Sequenced

6.1.10 Government Regulators Jump In

6.2 Review of the Hot Development Areas in Next-Generation Genome Sequencing

6.2.1 Next-Generation Sequencing Systems May Replace CE Sequencing and Enable New Applications

Table 19. Applications Unique to Next-Generation Sequencers

6.2.2 Automated Sample Preparation

6.2.3 Microarray Capture Method Front-end for Targeted Sequencing

6.3 Large-Scale International Sequencing Projects Use Next-Generation Sequencing

6.3.1 Scientists Want to Sequence 100,000’s of Humans to Enable Personal Medicine

6.3.2 The 1,000 Genomes Project

6.3.3 Human Microbiome Project

6.3.4 The Cancer Genome Atlas (US)

6.3.5 The Wellcome Trust Sanger Institute’s Cancer Genome Project

6.3.6 The Yanhuang Project

6.4 Next-Generation Genome Sequencing Business Opportunities or Implications

6.4.1 Opportunities or Implications for the US

Table 20. Opportunities or Implications for the US(Region, Opportunity Area, Comment)

6.4.2 Opportunities or Implications for Europe

Table 21. Opportunities or Implications for Europe(Region, Opportunity Area, Comment)

6.4.3 Opportunities or Implications for Japan and Asia (Region, Opportunity Area, Comment)

7. Appendix

7.1 Glossary

7.2 Key Genome Sequencing Centers